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pgl3 basic  (Addgene inc)


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    Structured Review

    Addgene inc pgl3 basic
    Pgl3 Basic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+control+vector/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pm41713257-68-22-27
    Average 93 stars, based on 48 article reviews
    pgl3 basic - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into the HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; https://www.addgene.org/212937/ ; RRID: Addgene_212937), expresses firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, 32°C, or 30°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures.
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into the HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instruc tions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; https://www.addgene.org/212937/; RRID: Addgene_212937), expresses firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, 32°C, or 30°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14.
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Control:

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: Coronary Artery Disease Risk Variant rs6903956 Links to Endothelial Dysfunction via PHACTR1 Regulation
    Article Snippet: .. The 519 bp ADTRP regulatory region flanking rs6903956 (-789 bp to +724 bp) (chr6:11,774,037–11,774,555) from Luo et al. was cloned into the pGL3 Control Vector (Addgene, Plasmid #212937) using KpnI and XhoI, upstream of the SV40 promoter and firefly luciferase coding region. .. Additionally, the same 519 bp region was cloned into the pGL3 Basic Vector (Addgene, Plasmid #212936) using XhoI and HindIII.

    Article Title: Endoplasmic reticulum stress drives proteinuria-induced kidney lesions via Lipocalin 2
    Article Snippet: .. In brief, subconfluent mIMCD-3 cells were co-transfected with pGL3 control vector or pGL3–24p3/Lcn2-Luc containing murine Lcn2 gene promoter (Addgene Inc., ID 25463) in combination with plasmids expressing protein of interest. pRL-SV40 Renilla control vector (Promega) was co-transfected in each condition. .. The luciferase activity was measured 36 h after transfection using a dual-luciferase reporter assay system (Promega) as recommended.

    Article Title: Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into the HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; https://www.addgene.org/212937/ ; RRID: Addgene_212937), expresses firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, 32°C, or 30°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures.
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into the HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instruc tions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; https://www.addgene.org/212937/; RRID: Addgene_212937), expresses firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, 32°C, or 30°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14.
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Plasmid Preparation:

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: Coronary Artery Disease Risk Variant rs6903956 Links to Endothelial Dysfunction via PHACTR1 Regulation
    Article Snippet: .. The 519 bp ADTRP regulatory region flanking rs6903956 (-789 bp to +724 bp) (chr6:11,774,037–11,774,555) from Luo et al. was cloned into the pGL3 Control Vector (Addgene, Plasmid #212937) using KpnI and XhoI, upstream of the SV40 promoter and firefly luciferase coding region. .. Additionally, the same 519 bp region was cloned into the pGL3 Basic Vector (Addgene, Plasmid #212936) using XhoI and HindIII.

    Article Title: Endoplasmic reticulum stress drives proteinuria-induced kidney lesions via Lipocalin 2
    Article Snippet: .. In brief, subconfluent mIMCD-3 cells were co-transfected with pGL3 control vector or pGL3–24p3/Lcn2-Luc containing murine Lcn2 gene promoter (Addgene Inc., ID 25463) in combination with plasmids expressing protein of interest. pRL-SV40 Renilla control vector (Promega) was co-transfected in each condition. .. The luciferase activity was measured 36 h after transfection using a dual-luciferase reporter assay system (Promega) as recommended.

    Article Title: Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into the HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; https://www.addgene.org/212937/ ; RRID: Addgene_212937), expresses firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, 32°C, or 30°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures.
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into the HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instruc tions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; https://www.addgene.org/212937/; RRID: Addgene_212937), expresses firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, 32°C, or 30°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14.
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Luciferase:

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: Coronary Artery Disease Risk Variant rs6903956 Links to Endothelial Dysfunction via PHACTR1 Regulation
    Article Snippet: .. The 519 bp ADTRP regulatory region flanking rs6903956 (-789 bp to +724 bp) (chr6:11,774,037–11,774,555) from Luo et al. was cloned into the pGL3 Control Vector (Addgene, Plasmid #212937) using KpnI and XhoI, upstream of the SV40 promoter and firefly luciferase coding region. .. Additionally, the same 519 bp region was cloned into the pGL3 Basic Vector (Addgene, Plasmid #212936) using XhoI and HindIII.

    Article Title: Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into the HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; https://www.addgene.org/212937/ ; RRID: Addgene_212937), expresses firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, 32°C, or 30°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures.
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into the HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instruc tions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; https://www.addgene.org/212937/; RRID: Addgene_212937), expresses firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, 32°C, or 30°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14.
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Clone Assay:

    Article Title: Coronary Artery Disease Risk Variant rs6903956 Links to Endothelial Dysfunction via PHACTR1 Regulation
    Article Snippet: .. The 519 bp ADTRP regulatory region flanking rs6903956 (-789 bp to +724 bp) (chr6:11,774,037–11,774,555) from Luo et al. was cloned into the pGL3 Control Vector (Addgene, Plasmid #212937) using KpnI and XhoI, upstream of the SV40 promoter and firefly luciferase coding region. .. Additionally, the same 519 bp region was cloned into the pGL3 Basic Vector (Addgene, Plasmid #212936) using XhoI and HindIII.

    Expressing:

    Article Title: Endoplasmic reticulum stress drives proteinuria-induced kidney lesions via Lipocalin 2
    Article Snippet: .. In brief, subconfluent mIMCD-3 cells were co-transfected with pGL3 control vector or pGL3–24p3/Lcn2-Luc containing murine Lcn2 gene promoter (Addgene Inc., ID 25463) in combination with plasmids expressing protein of interest. pRL-SV40 Renilla control vector (Promega) was co-transfected in each condition. .. The luciferase activity was measured 36 h after transfection using a dual-luciferase reporter assay system (Promega) as recommended.



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